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LearnM810
antibody from Invitrogen Antibodies
Targeting: CCL13
CKb10, MCP-4, MGC17134, NCC-1, SCYA13, SCYL1
ELISA
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- M810 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCP-4 Monoclonal Antibody (8C12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- The M810 MCP-4 antibody (clone 8C12) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M809 (biotinylated conjugate of clone 3G4). The M810 MCP-4 antibody (biotinylated conjugate of clone 8C12) has also been used successfully in a sandwich ELISA as a detection antibody when paired with M809 (clone 3G4) as the coating antibody.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C12
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of an anti-human MCP-4 monoclonal antibody (Product # M810) was performed by loading 100 µL per well of antibody (Product # M810) at a concentration of 2 µg/mL overnight at room temperature. The plate was washed 3 times with ELISA Wash Buffer (Product # N503), and 100 µL of recombinant human MCP-4/CCL13 was added to wells in duplicate at 2000, 1000, 500, 250, 125, 62.5, 31.25, and 0pg/mL concentrations and the samples were incubated for 2 hours at room temperature. The plate was washed, then incubated with 100 µL per well of a biotinylated MCP-4 monoclonal antibody (Product # M809, biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Product # 21435) at a concentration of 0.13 µg/mL for 1 hour at room temperature, followed by 100 µL per well of Streptavidin-HRP (Product # N504) at a dilution of 1:16,000 for 30 minutes at room temperature. Detection was performed by adding 100 µL of Ultra TMB substrate (Product # 34028) per well and incubating for 30 minutes at room temperature in the dark. The plate was then stopped with 100 µL per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of an anti-human MCP-4 monoclonal antibody (Product # M809) was performed by loading 100 µL per well of antibody (Product # M809) at a concentration of 2 µg/mL overnight at room temperature. The plate was washed 3 times with ELISA Wash Buffer (Product # N503), and 100 µL of recombinant human MCP-4/CCL13 was added to wells in duplicate at 2000, 500, 125, 31.25, 7.813, and 0pg/mL concentrations and the samples were incubated for 2 hours at room temperature. The plate was washed, then incubated with 100 µL per well of a biotinylated MCP-4 monoclonal antibody (Product # M810, biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Product # 21435) at a concentration of 0.13 µg/mL for 1 hour at room temperature, followed by 100 µL per well of Streptavidin-HRP (Product # N504) at a dilution of 1:16,000 for 30 minutes at room temperature. Detection was performed by adding 100 µL of Ultra TMB substrate (Product # 34028) per well and incubating for 30 minutes at room temperature in the dark. The plate was then stopped with 100 µL per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
