Explore
Validate
Learn500-P04BT-1MG
antibody from Invitrogen Antibodies
Targeting: CCL13
CKb10, MCP-4, MGC17134, NCC-1, SCYA13, SCYL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P04BT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCP-4 Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human MCP-4 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MCP-4 (CCL13) (500-P04) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human MCP-4. Western Blot: To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P04BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Mateo T, Abu Nabah YN, Abu Taha M, Mata M, Cerdá-Nicolás M, Proudfoot AE, Stahl RA, Issekutz AC, Cortijo J, Morcillo EJ, Jose PJ, Sanz MJ
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MCP-4 by Western Blot analysis MCP-4 Polyclonal Antibody, Biotin (Product # 500-P04BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MCP-4 by Western Blot analysis MCP-4 Polyclonal Antibody, Biotin (Product # 500-P04BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MCP-4 by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of MCP-4 Polyclonal Antibody, Biotin (Product # 500-P04BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MCP-4 Polyclonal Antibody (Product # 500-P04-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human MCP-4.
- Conjugate
- Biotin
