TA800102

antibody from OriGene

Targeting: PSMA6 IOTA, MGC22756, MGC2333, MGC23846, p27K, PROS27

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA800102

Provider

OriGene

Proper citation

OriGene Cat#TA800102, RRID:AB_2625225

Product name

PSMA6 (Proteasome 20S alpha 6) mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Antibody type

Monoclonal

Description

PSMA6 (Proteasome 20S alpha 6) mouse monoclonal antibody, clone OTI5H3 (formerly 5H3)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

PSMA6

Isotype

IgG

Antibody clone number

OTI5H3

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (RC209976, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PSMA6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800102)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209976 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PSMA6 antibody(TA800102), and then analyzed by flow cytometry.

Validation comment

FC