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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-40640 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOX18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: RTAPPAAPLA GLYYGTLGTP GPYPGPLSPP PEAPPLESAE PLGPAADLWA Sequence homology: Cow: 86%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SOX18 in lymphatic endothelial cell tissue. Samples were incubated with SOX18 polyclonal antibody (Product # PA5-40640) using a dilution of 1:50 up to 1:500 followed by secondary at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SOX18 in paraffin embedded human kidney tissue. Samples were incubated with SOX18 polyclonal antibody (Product # PA5-40640) using a dilution of 4.0-8.0 µg/mL. Cellular data: Epithelial cells of renal tubule. Magnification: 400X.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface Plasmon Resonance of SOX18 polyclonal antibody (Product # PA5-40640). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 µg/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
