500-P110-1MG

antibody from Invitrogen Antibodies

Targeting: CCL1 I-309, P500, SCYA1, SISe, TCA3

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P110-1MG

Provider

Invitrogen Antibodies

Product name

CCL1 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: SKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human I-309 (CCL1). Anti-Human I-309 (CCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human I-309 (CCL1) matrix. Sandwich ELISA: To detect Human I-309 (CCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human I-309 (CCL1) (500-P110Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-309 (CCL1). Western Blot: To detect Human I-309 (CCL1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human I-309 (CCL1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

1 mg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

Structure/function relationships of CCR8 agonists and antagonists. Amino-terminal extension of CCL1 by a single amino acid generates a partial agonist.

Fox JM, Najarro P, Smith GL, Struyf S, Proost P, Pease JE
The Journal of biological chemistry 2006 Dec 1;281(48):36652-61

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CCR4 ligands are up-regulated in the airways of atopic asthmatics after segmental allergen challenge.

Pilette C, Francis JN, Till SJ, Durham SR
The European respiratory journal 2004 Jun;23(6):876-84

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Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes.

Annunziato F, Cosmi L, Liotta F, Lazzeri E, Manetti R, Vanini V, Romagnani P, Maggi E, Romagnani S
The Journal of experimental medicine 2002 Aug 5;196(3):379-87

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human I-309 (CCL1) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL1 Polyclonal Antibody (Product # 500-P110-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL1 Polyclonal Antibody, Biotin (Product # 500-P110BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human I-309 (CCL1).