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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB4138 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse S100A4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse S100A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse S100A6, 8, 9, 10, 13, 16, recombinant human (rh) S100A1, 2, 7, 11, or rhS100P is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P07091- Isotype
- IgG
- Antibody clone number
- 438709
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Functional expression, characterization and application of the human S100A4 protein.
Wang D, Zhang J, Liu Z, Chen Y, Xu C, Zhang Z, Liu X, Wu L, Zhou X, Meng X, Li H, Liu H, Jiang Z, Wang T
Molecular medicine reports 2015 Jan;11(1):175-81
Molecular medicine reports 2015 Jan;11(1):175-81
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse S100A4 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A4 Monoclonal Antibody (Catalog # MAB4138) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A4 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
