DM1222

antibody from OriGene

Targeting: S100A4 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1222

Provider

OriGene

Product name

S100A4 mouse monoclonal antibody, clone NJ-4F3, Purified

Antibody type

Monoclonal

Description

S100A4 mouse monoclonal antibody, clone NJ-4F3, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

S100A4

Isotype

IgG

Antibody clone number

NJ-4F3

Vial size

100 µg

Concentration

1.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

SDS-PAGE analysis of purified NJ-4F3 Antibody : Lane 1: Molecular weight marker. Lane 2: 2 ug of purified NJ-4F3 antibody. Proteins were separated by CGE (Capillary Gel Electrophoresis, Agilent 2100 Bioanalyzer). Internal Control bands (240 kDa / 7 kDa / 4.5 kDa).

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

FACS analysis of BOSC23 cells using NJ-4F3 Antibody DM122:. BOSC23 cells were transiently transfected with anexpression vector encoding either S100A4 (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with S100A4 trans-fected cells.

Validation comment

FC