M01217-1

antibody from Boster Biological Technology

Targeting: S100A4 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M01217-1

Provider

Boster Biological Technology

Product name

Anti-S100A4 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for S100A4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat S100A4 information: Molecular Weight: 11729 MW; Tissue Specificity: Ubiquitously expressed.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

GFG-19

Vial size

100ug/vial

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Design and Synthesis of Matrine Derivatives as Novel Anti-Pulmonary Fibrotic Agents via Repression of the TGFβ/Smad Pathway.

Li L, Ma L, Wang D, Jia H, Yu M, Gu Y, Shang H, Zou Z
Molecules (Basel, Switzerland) 2019 Mar 20;24(6)

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Dihydroartemisinin attenuates renal fibrosis through regulation of fibroblast proliferation and differentiation.

Zhang B, Liu P, Zhou Y, Chen Z, He Y, Mo M, Dai G, Xia W, Du Y, Liu Y, Chen X
Life sciences 2019 Apr 15;223:29-37

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of S100A4 expression in A375 cell lysate (M01217-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A4 monoclonal antibody (Catalog # M01217-1) overnight at 4

Additional image