V8068

antibody from NSJ Bioreagents

Targeting: S100A4 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8068

Provider

NSJ Bioreagents

Product name

Recombinant FSP1 Antibody / S100A4

Antibody type

Monoclonal

Description

This highly specific Recombinant FSP1 antibody is suitable for use in Flow cytometry/Western blot/Immunofluorescence/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

rS100A4/1481

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the recombinant FSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HeLa and A549 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481); Red=isotype control, Blue= FSP1 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.