Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- V8068 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- S100A4 Antibody / FSP1
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- rS100A4/1481
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant FSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481); Red=isotype control, Blue= FSP1 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.