6275-MSM1-P0

antibody from Invitrogen Antibodies

Targeting: S100A4 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

6275-MSM1-P0

Provider

Invitrogen Antibodies

Product name

S100A4/Metastasin/Calvasculin Monoclonal Antibody (S100A4, 1481)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

S100A4, 1481

Vial size

20 µg

Concentration

200 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HeLa, A549 and A375 cell lysates using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HeLa cell lysate using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human placenta using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of T98G cells using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of A549 cells using S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Analysis of Protein Array containing more than 19,000 full-length human proteins S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÔøΩs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÔøΩs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SDS-PAGE Analyis Purified S100A4, Metastasin, Calvasculin Monoclonal Antibody (Product # 6275-MSM1-P1). Confirmation of Purity and Integrity of Antibody.