HPA007973

S100A4 antibody from Atlas Antibodies

18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Western blot

Enhanced validation

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for HPA007973

Antibody Data

Product number

HPA007973

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007973, RRID:AB_1079858

Product name

Anti-S100A4

Provider product page

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELK
ELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEV
DFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling
den Boon J, Pyeon D, Wang S, Horswill M, Schiffman M, Sherman M, Zuna R, Wang Z, Hewitt S, Pearson R, Schott M, Chung L, He Q, Lambert P, Walker J, Newton M, Wentzensen N, Ahlquist P
Proceedings of the National Academy of Sciences 2015 June;112(25)

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Decidual PTEN expression is required for trophoblast invasion in the mouse
Lague M, Detmar J, Paquet M, Boyer A, Richards J, Adamson S, Boerboom D
AJP: Endocrinology and Metabolism 2010 November;299(6)

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-S100A4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and S100A4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418991).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and pancreas tissues using Anti-S100A4 antibody. Corresponding S100A4 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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