HPA007973

S100A4 antibody from Atlas Antibodies

18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA007973

SELECT THIS
ANTIBODY

PERFORM KNOCKDOWN
OR KNOCKOUT

SUBMIT POSITIVE
RESULTS

GET
REWARDED

Antibody Data

Product number

HPA007973

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007973, RRID:AB_888795

Product name

Anti-S100A4

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELK
ELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEV
DFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Molecular transitions from papillomavirus infection to cervical precancer and cancer: Role of stromal estrogen receptor signaling
den Boon J, Pyeon D, Wang S, Horswill M, Schiffman M, Sherman M, Zuna R, Wang Z, Hewitt S, Pearson R, Schott M, Chung L, He Q, Lambert P, Walker J, Newton M, Wentzensen N, Ahlquist P
Proceedings of the National Academy of Sciences 2015 June;112(25)

---

Decidual PTEN expression is required for trophoblast invasion in the mouse
Lague M, Detmar J, Paquet M, Boyer A, Richards J, Adamson S, Boerboom D
AJP: Endocrinology and Metabolism 2010 November;299(6)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and S100A4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418991).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and pancreas tissues using Anti-S100A4 antibody. Corresponding S100A4 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more