Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4146 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Annexin A4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse, and rat Annexin A4 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A7, A8, A9, A10, A11or A13.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System.
Transcription factor induced conversion of human fibroblasts towards the hair cell lineage.
Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells.
Sayyid ZN, Wang T, Chen L, Jones SM, Cheng AG
Cell reports 2019 Jul 9;28(2):312-324.e4
Cell reports 2019 Jul 9;28(2):312-324.e4
Transcription factor induced conversion of human fibroblasts towards the hair cell lineage.
Duran Alonso MB, Lopez Hernandez I, de la Fuente MA, Garcia-Sancho J, Giraldez F, Schimmang T
PloS one 2018;13(7):e0200210
PloS one 2018;13(7):e0200210
Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells.
Liu XP, Koehler KR, Mikosz AM, Hashino E, Holt JR
Nature communications 2016 May 24;7:11508
Nature communications 2016 May 24;7:11508
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Annexin A4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Annexin A4 in Human Kidney. Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human/Mouse/Rat Annexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4146) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes, cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.