Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-34769 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A4 Recombinant Rabbit Monoclonal Antibody (JG37-83)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Human liver tissue lysate, A549, HepG2, SiHa, human colon cancer tissue, human kidney tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG37-83
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin IV in human liver tissue lysate. Samples were incubated with Annexin IV monoclonal antibody (Product # MA5-34769), at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin IV in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Annexin IV monoclonal antibody (Product # MA5-34769), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin IV in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Annexin IV monoclonal antibody (Product # MA5-34769), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin IV in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Annexin IV monoclonal antibody (Product # MA5-34769), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Annexin IV in paraffin-embedded human kidney tissue. Samples were incubated with Annexin IV monoclonal antibody (Product # MA5-34769), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Annexin IV in paraffin-embedded human colon cancer tissue. Samples were incubated with Annexin IV monoclonal antibody (Product # MA5-34769), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Annexin IV in HepG2 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Annexin IV monoclonal antibody (Product # MA5-34769) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.