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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27871 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin A4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Annexin A4 Polyclonal Antibody (Product # PA5-27871). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. Annexin A4 Polyclonal Antibody . Annexin A4 Polyclonal Antibody (Product # PA5-27871) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Annexin A4 in methanol-fixed HeLa cells using an Annexin A4 polyclonal antibody (Product # PA5-27871) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Annexin A4 Polyclonal Antibody detects ANXA4 protein at cytosol and membrane on D54MG xenograft by immunohistochemical analysis. Sample: Paraffin-embedded D54MG xenograft. Annexin A4 Polyclonal Antibody (Product # PA5-27871) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Annexin IV was performed in HepG2 whole cell extracts using 5 µg of Annexin A4 Polyclonal Antibody (Product # PA5-27871). Samples were transferred to a membrane and probed with Annexin A4 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
