Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4146 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Annexin A4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse, and rat Annexin A4 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A7, A8, A9, A10, A11or A13.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_001144
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Transcription factor induced conversion of human fibroblasts towards the hair cell lineage.
Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells.
Duran Alonso MB, Lopez Hernandez I, de la Fuente MA, Garcia-Sancho J, Giraldez F, Schimmang T
PloS one 2018;13(7):e0200210
PloS one 2018;13(7):e0200210
Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells.
Liu XP, Koehler KR, Mikosz AM, Hashino E, Holt JR
Nature communications 2016 May 24;7:11508
Nature communications 2016 May 24;7:11508
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Annexin A4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Annexin A4 in Human Kidney. Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Human/Mouse/Rat Annexin A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4146) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes, cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.