PA5-41867

antibody from Invitrogen Antibodies

Targeting: LARP7 DKFZP564K112, HDCMA18P, PIP7S

Antibody data

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Validation data

Product number: PA5-41867 - Provider product page
Provider: Invitrogen Antibodies
Product name: LARP7 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Peptide sequence: WQKILVDRQA KLNQPREKKR GTEKLITKAE KIRLAKTQQA SKHIRFSEYD
Concentration: 0.5 mg/mL

LARP7 protein structure - PA5-41867 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LARP7 (same in Drosophila) using LARP7 Rabbit Polyclonal Antibody (Product # PA5-41867) was performed by loading 10 mg of each cell lysates: HeLa (lane 1) and Drosophila S2 (lane 2) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% SDS-PAGE gel. Proteins were transferred to PVDF membrane (Product # 88518) at 30V for 60 min on ice. Membrane was blocked in 5% Milk in PBST for 30 min at room temperature. The primary LARP7 polyclonal antibody (Product # PA5-41867) was used at a dilution of 1:1000 in 5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 for 1 hr. Chemiluminescent detection was performed using ECL substrate. Strong band ~72 kDa was detected at HeLa cells and three at Drosophila (one is corresponding with predicted ~67 kDa band for Drosophila LARP7). Data courtesy of Dr. Fondufe-Mittendorf’s lab at University of Kentucky.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of LARP7 (same in Drosophila) using LARP7 Rabbit Polyclonal Antibody (Product # PA5-41867) was performed by loading 10 mg of each cell lysates: HeLa (lane 1) and Drosophila S2 (lane 2) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 10% SDS-PAGE gel. Proteins were transferred to PVDF membrane (Product # 88518) at 30V for 60 min on ice. Membrane was blocked in 5% Milk in PBST for 30 min at room temperature. The primary LARP7 polyclonal antibody (Product # PA5-41867) was used at a dilution of 1:1000 in 5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000 for 1 hr. Chemiluminescent detection was performed using ECL substrate. Strong band ~72 kDa was detected at HeLa cells and three at Drosophila (one is corresponding with predicted ~67 kDa band for Drosophila LARP7). Data courtesy of Dr. Fondufe-Mittendorf’s lab at University of Kentucky.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Hela whole cell lysate using an anti-LARP7 polyclonal antibody (Product # PA5-41867). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of human HeLa cell lysate using an anti-LARP7 polyclonal antibody (Product # PA5-41867).