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Learn12-1339-41
antibody from Invitrogen Antibodies
Targeting: PROM1
AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 12-1339-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD133 (Prominin-1) Monoclonal Antibody (EMK08), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others. The binding of the EMK08 antibody does not block the binding of another anti-human CD133 antibody, TMP4 (cat. 12-1338) indicating that they recognize distinct epitopes. Applications Reported: This EMK08 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EMK08 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- EMK08
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells.
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
Guo P, Wang J, Gao W, Liu X, Wu S, Wan B, Xu L, Li Y
Molecular medicine reports 2018 Aug;18(2):1323-1334
Molecular medicine reports 2018 Aug;18(2):1323-1334
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
Chen Q, Khoury M, Chen J
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 22;106(51):21783-8
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 22;106(51):21783-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-9459-42) and Mouse IgG2b K Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human CD133 PE (right). Total viable cells were used for analysis.
- Conjugate
- Yellow dye
