17-1339-41
antibody from Invitrogen Antibodies
Targeting: PROM1
AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4
Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 17-1339-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD133 (Prominin-1) Monoclonal Antibody (EMK08), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others. The binding of the EMK08 antibody does not block the binding of another anti-human CD133 antibody, TMP4 (Product # 12-1338) indicating that they recognize distinct epitopes. Applications Reported: This EMK08 antibody has been reported for use in flow cytometric analysis. Applications Tested: This EMK08 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EMK08
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis.
Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells.
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
A human colon cancer cell capable of initiating tumour growth in immunodeficient mice.
Identification of human brain tumour initiating cells.
AC133, a novel marker for human hematopoietic stem and progenitor cells.
Ye W, Ni Z, Yicheng S, Pan H, Huang Y, Xiong Y, Liu T
International journal of oncology 2019 Dec;55(6):1296-1312
International journal of oncology 2019 Dec;55(6):1296-1312
Salvianolic acid B reverses multidrug resistance in nude mice bearing human colon cancer stem cells.
Guo P, Wang J, Gao W, Liu X, Wu S, Wan B, Xu L, Li Y
Molecular medicine reports 2018 Aug;18(2):1323-1334
Molecular medicine reports 2018 Aug;18(2):1323-1334
Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.
Chen Q, Khoury M, Chen J
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 22;106(51):21783-8
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 22;106(51):21783-8
A human colon cancer cell capable of initiating tumour growth in immunodeficient mice.
O'Brien CA, Pollett A, Gallinger S, Dick JE
Nature 2007 Jan 4;445(7123):106-10
Nature 2007 Jan 4;445(7123):106-10
Identification of human brain tumour initiating cells.
Singh SK, Hawkins C, Clarke ID, Squire JA, Bayani J, Hide T, Henkelman RM, Cusimano MD, Dirks PB
Nature 2004 Nov 18;432(7015):396-401
Nature 2004 Nov 18;432(7015):396-401
AC133, a novel marker for human hematopoietic stem and progenitor cells.
Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW
Blood 1997 Dec 15;90(12):5002-12
Blood 1997 Dec 15;90(12):5002-12
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45 FITC (Product # 11-0459-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human CD133 APC (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Identification of colon CSCs. (A) Flow cytometry analysis and quantification of CD133 expression in spheroid cells and corresponding parental cells. Cells in Q2-2 correspond to CD133-positive cells. Results are representative images of three independent experiments. **P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Proportion of CD44 + /CD133 + cancer stem cells in ovarian cancer tissues of each patient by flow cytometry.