A01767-3

antibody from Boster Biological Technology

Targeting: PROM1 AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A01767-3

Provider

Boster Biological Technology

Product name

Anti-PROM1 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for PROM1 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Identification of miRNA signature associated with BMP2 and chemosensitivity of TMZ in glioblastoma stem-like cells.

Guo X, Luo Z, Xia T, Wu L, Shi Y, Li Y
Genes & diseases 2020 Sep;7(3):424-439

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Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.

Jiang T, Zhao J, Yu S, Mao Z, Gao C, Zhu Y, Mao C, Zheng L
Biomaterials 2019 Jan;188:130-143

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CD146 is highly expressed in glioma stem cells and acts as a cell cycle regulator.

Yawata T, Higashi Y, Kawanishi Y, Nakajo T, Fukui N, Fukuda H, Ueba T
Journal of neuro-oncology 2019 Aug;144(1):21-32

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Identification and differentiation therapy strategy of pterygium in vitro.

Hu X, Tandra N, Zhang Z, Gong A, Chen J, Li Y, Chen Q, Xu W, Qian H
American journal of translational research 2018;10(8):2619-2627

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Effects of 5-hydroxy-4'-nitro-7-propionyloxy-genistein on inhibiting proliferation and invasion via activating reactive oxygen species in human ovarian cancer A2780/DDP cells.

Bai J, Yang BJ, Luo X
Oncology letters 2018 Apr;15(4):5227-5235

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Isolation and Molecular Characterization of Amniotic Fluid-Derived Mesenchymal Stem Cells Obtained from Caesarean Sections.

Spitzhorn LS, Rahman MS, Schwindt L, Ho HT, Wruck W, Bohndorf M, Wehrmeyer S, Ncube A, Beyer I, Hagenbeck C, Balan P, Fehm T, Adjaye J
Stem cells international 2017;2017:5932706

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β-elemene inhibits stemness, promotes differentiation and impairs chemoresistance to temozolomide in glioblastoma stem-like cells.

Zhu TZ, Li XM, Luo LH, Song ZQ, Gao X, Li ZQ, Su JY, Liang GB
International journal of oncology 2014 Aug;45(2):699-709

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of PROM1 using anti-PROM1 antibody (A01767-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 97KD. The expected band size for PROM1 is at 97KD.

Additional image