PAB21788

antibody from Abnova Corporation

Targeting: PLEKHF1 APPD, MGC4090, PHAFIN1, ZFYVE15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21788

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21788, RRID:AB_10965089

Product name

PLEKHF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PLEKHF1.

Antigen sequence

MVDHLANTEINSQRIAAVESCFGASGQPLALPGRV
LLGEGVLTKECRKKAKPRIFFLFNDILVYGSIVLN
KRKYRSQHIIPLEEVTLELLPETLQAKNRWMIKTA
KKSFVVSAASATERQEWISHIEE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PLEKHF1 polyclonal antibody (Cat # PAB21788).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with PLEKHF1 polyclonal antibody (Cat # PAB21788) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human hippocampus with PLEKHF1 polyclonal antibody (Cat # PAB21788) shows strong cytoplasmic positivity in astrocyte-like cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)