MAB2177

antibody from Abnova Corporation

Targeting: IP6K2 IHPK2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2177

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB2177, RRID:AB_1678841

Product name

IP6K2 monoclonal antibody, clone 4F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against full length recombinant IP6K2.

Isotype

IgG

Antibody clone number

4F10

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Effect of gamma-irradiation and IFN-beta on IP6K2 protein levels.(a) NIH:OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 (white bars) or 2 Gy (gray bars) irradiation and replated in complete medium.At 8 and 16 h after plating, cells were lysed and subject to Western blot analysis using IP6K2 monoclonal antibody, clone 4F10 (Cat # MAB2177).To control for loading, blots were probed with anti-actin antibody; bands were digitized and quantified.IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1.Purified rIP6K2 was included as a positive control (lane 1).Numbers to right of blots indicate MW markers.Only relevant portion of the blots are shown.(b) NIH-OVAR-3 cells were grown in the presence of 200 U/ml IFN-beta for 0-48 h and then subject to Western blot analysis and quantified as above.