Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C759923 - Provider product page
- Provider
- LSBio
- Product name
- IP6K2 Antibody (clone 4F10) LS-C759923
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4F10
- Storage
- One year when stored at -20°C. Avoid multiple freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
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- Experimental details
- Effect of ?-irradiation and IFN-ß on IP6K2 protein levels. (a) NIH-OVCAR-3 cells growing at 75% confluence were trypsinized, suspended in 1 ml PBS, and subject to 0, 1 (white bars) or Gy (gray bars) irradiation and replated in complete medium. At 8 and 16 h after plating, cells were harvested, lysed and subject to Western blot analysis using monoclonal anti-IP6K2 antibodies. To control for loading, blots were probed with anti-actin antibodies; bands were digitized and quantified. IP6K2 signal intensity was normalized to actin, then expressed as fold induction, with unirradiated cells representing a protein level of 1. Purified rIP6K2 was included as a positive control (lane 1). Numbers to right of blots indicate MW markers. Only relevant portion of the blots are shown. (b) NIH-OVCAR-3 cells were grown in the presence of 200 U/ml IFN-ß for 0-48 h and then subject to Western blot analysis and quantified as above.