Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-53850 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NLE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: TIKVWRAHDG VLCRTLQGHG HWVNTMALST DYALRTGAFE PAEASVNPQD LQGSLQELKE RALSRYNLVR GQGPERLVSG SDDFTL Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorecent analysis of NLE1 in human cell line A-431 using NLE1 Polyclonal Antibody (Product # PA5-53850). Staining shows positivity in nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLE1 in human cerebral cortex using NLE1 Polyclonal Antibody (Product # PA5-53850) shows moderate nucleoli positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLE1 in human fallopian tube using NLE1 Polyclonal Antibody (Product # PA5-53850) shows moderate nucleoli positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLE1 in human testis using NLE1 Polyclonal Antibody (Product # PA5-53850) shows moderate nucleoli and nuclear positivity in subset of cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NLE1 in human skeletal muscle using NLE1 Polyclonal Antibody (Product # PA5-53850) shows no nuclear positivity in myocytes as expected.