12175-1-AP

antibody from Proteintech Group

Targeting: OXCT1 OXCT, SCOT

Provider product page for 12175-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [44]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 12175-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12175-1-AP, RRID:AB_2157444
Product name: OXCT1 antibody
Antibody type: Polyclonal
Description: KD/KO validated OXCT1 antibody (Cat. #12175-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

OXCT1 protein structure - 12175-1-AP shown in red.

Submitted references The ketogenic diet does not improve cardiac function and blunts glucose oxidation in ischaemic heart failure.

Ho KL, Karwi QG, Wang F, Wagg C, Zhang L, Panidarapu S, Chen B, Pherwani S, Greenwell AA, Oudit GY, Ussher JR, Lopaschuk GD
Cardiovascular research 2024 Sep 2;120(10):1126-1137

Targeting OXCT1-mediated ketone metabolism reprograms macrophages to promote antitumor immunity via CD8(+) T cells in hepatocellular carcinoma.

Zhu CX, Yan K, Chen L, Huang RR, Bian ZH, Wei HR, Gu XM, Zhao YY, Liu MC, Suo CX, Li ZK, Yang ZY, Lu MQ, Hua XF, Li L, Zhao ZB, Sun LC, Zhang HF, Gao P, Lian ZX
Journal of hepatology 2024 Oct;81(4):690-703

Widespread stable noncanonical peptides identified by integrated analyses of ribosome profiling and ORF features.

Yang H, Li Q, Stroup EK, Wang S, Ji Z
Nature communications 2024 Mar 2;15(1):1932

OXCT1 regulates hippocampal neurogenesis and alleviates cognitive impairment via the Akt/GSK-3β/β-catenin pathway after subarachnoid hemorrhage.

Qiu JY, Gao SQ, Chen YS, Wang X, Zhuang YS, Miao SH, Zheng XB, Zhao R, Sun Y, Zhou ML
Brain research 2024 Mar 15;1827:148758

Acetoacetate and d- and l-β-hydroxybutyrate have distinct effects on basal and insulin-stimulated glucose uptake in L6 skeletal muscle cells.

Khouri H, Roberge M, Ussher JR, Aguer C
American journal of physiology. Cell physiology 2024 Jun 1;326(6):C1710-C1720

Development of an integrated and project-based laboratory course in upper-level biochemistry and molecular biology.

Liu Y, Tu S, Hu X, Xiong X, Pan Z, Liu Z, Zhu W, Luo D, Cui X, Huang C, Xie C
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology 2024 Jul-Aug;52(4):411-425

Ketone flux through BDH1 supports metabolic remodeling of skeletal and cardiac muscles in response to intermittent time-restricted feeding.

Williams AS, Crown SB, Lyons SP, Koves TR, Wilson RJ, Johnson JM, Slentz DH, Kelly DP, Grimsrud PA, Zhang GF, Muoio DM
Cell metabolism 2024 Feb 6;36(2):422-437.e8

OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB.

Ma W, Sun Y, Yan R, Zhang P, Shen S, Lu H, Zhou Z, Jiang Z, Ye L, Mao Q, Xiong N, Jia W, Sun L, Gao P, Zhang H
Molecular cell 2024 Feb 1;84(3):538-551.e7

β-hydroxybutyrate impairs bovine oocyte maturation via pyruvate dehydrogenase (PDH) associated energy metabolism abnormality.

Zhang KY, Guo J, Zhan CL, Yuan CS, Min CG, Li ZQ, Liu HY, Wang J, Zhao J, Lu WF, Ma X
Frontiers in pharmacology 2023;14:1243243

Tandem mass tag-based quantitative proteomic analysis identification of succinylation related proteins in pathogenesis of thoracic aortic aneurysm and aortic dissection.

Zhang Y, Zhang H, Wang H, Wang C, Yang P, Lu C, Liu Y, Xu Z, Xie Y, Hu J
PeerJ 2023;11:e15258

Ketolysis drives CD8(+) T cell effector function through effects on histone acetylation.

Luda KM, Longo J, Kitchen-Goosen SM, Duimstra LR, Ma EH, Watson MJ, Oswald BM, Fu Z, Madaj Z, Kupai A, Dickson BM, DeCamp LM, Dahabieh MS, Compton SE, Teis R, Kaymak I, Lau KH, Kelly DP, Puchalska P, Williams KS, Krawczyk CM, Lévesque D, Boisvert FM, Sheldon RD, Rothbart SB, Crawford PA, Jones RG
Immunity 2023 Sep 12;56(9):2021-2035.e8

Defective muscle ketone body oxidation disrupts BCAA catabolism by altering mitochondrial branched-chain aminotransferase.

Mechchate H, Abdualkader AM, Bernacchi JB, Gopal K, Tabatabaei Dakhili SA, Yang K, Greenwell AA, Kong X, Crawford PA, Al Batran R
American journal of physiology. Endocrinology and metabolism 2023 May 1;324(5):E425-E436

Neuroprotective mechanisms of OXCT1 via the SIRT3-SOD2 pathway after traumatic brain injury.

Zhuang YS, Wang X, Gao SQ, Miao SH, Li T, Gao CC, Han YL, Qiu JY, Zhou ML, Wang HD
Brain research 2023 Jun 1;1808:148324

Ketogenic diet modulates cardiac metabolic dysregulation in streptozocin-induced diabetic rats.

Trang NN, Lee TW, Kao YH, Chao TF, Lee TI, Chen YJ
The Journal of nutritional biochemistry 2023 Jan;111:109161

The Antipsychotic Dopamine 2 Receptor Antagonist Diphenylbutylpiperidines Improve Glycemia in Experimental Obesity by Inhibiting Succinyl-CoA:3-Ketoacid CoA Transferase.

Tabatabaei Dakhili SA, Greenwell AA, Yang K, Abou Farraj R, Saed CT, Gopal K, Chan JSF, Chahade JJ, Eaton F, Lee C, Velázquez-Martínez CA, Crawford PA, Glover JNM, Al Batran R, Ussher JR
Diabetes 2023 Jan 1;72(1):126-134

Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice.

Rome FI, Shobert GL, Voigt WC, Stagg DB, Puchalska P, Burgess SC, Crawford PA, Hughey CC
American journal of physiology. Endocrinology and metabolism 2023 Jan 1;324(1):E9-E23

Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.

Peng SL, Wang R, Zhou YL, Wei W, Zhong GH, Huang XT, Yang S, Liu QD, Liu ZG
Frontiers in immunology 2022;13:872910

Ketogenic diet uncovers differential metabolic plasticity of brain cells.

Düking T, Spieth L, Berghoff SA, Piepkorn L, Schmidke AM, Mitkovski M, Kannaiyan N, Hosang L, Scholz P, Shaib AH, Schneider LV, Hesse D, Ruhwedel T, Sun T, Linhoff L, Trevisiol A, Köhler S, Pastor AM, Misgeld T, Sereda M, Hassouna I, Rossner MJ, Odoardi F, Ischebeck T, de Hoz L, Hirrlinger J, Jahn O, Saher G
Science advances 2022 Sep 16;8(37):eabo7639

Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer.

Tang M, Xu H, Huang H, Kuang H, Wang C, Li Q, Zhang X, Ge Y, Song M, Zhang X, Wang Z, Ma C, Kang J, Zhang W, Wang Y, Zhang B, Zhang X, Chen Y, Cong M, Melino G, Wang X, Zhou F, Sun Q, Shi H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Oct;9(29):e2201992

Exogenous ketone ester administration attenuates systemic inflammation and reduces organ damage in a lipopolysaccharide model of sepsis.

Soni S, Martens MD, Takahara S, Silver HL, Maayah ZH, Ussher JR, Ferdaoussi M, Dyck JRB
Biochimica et biophysica acta. Molecular basis of disease 2022 Nov 1;1868(11):166507

Ketone body oxidation increases cardiac endothelial cell proliferation.

Weis EM, Puchalska P, Nelson AB, Taylor J, Moll I, Hasan SS, Dewenter M, Hagenmüller M, Fleming T, Poschet G, Hotz-Wagenblatt A, Backs J, Crawford PA, Fischer A
EMBO molecular medicine 2022 Apr 7;14(4):e14753

Pan-Cancer Analysis of Glycolytic and Ketone Bodies Metabolic Genes: Implications for Response to Ketogenic Dietary Therapy.

Qian L, Li Y, Cao Y, Meng G, Peng J, Li H, Wang Y, Xu T, Zhang L, Sun B, Li B, Yu D
Frontiers in oncology 2021;11:689068

Barth syndrome-related cardiomyopathy is associated with a reduction in myocardial glucose oxidation.

Greenwell AA, Gopal K, Altamimi TR, Saed CT, Wang F, Tabatabaei Dakhili SA, Ho KL, Zhang L, Eaton F, Kruger J, Al Batran R, Lopaschuk GD, Oudit GY, Ussher JR
American journal of physiology. Heart and circulatory physiology 2021 Jun 1;320(6):H2255-H2269

WHSC1 Promotes Cell Proliferation, Migration, and Invasion in Hepatocellular Carcinoma by Activating mTORC1 Signaling.

Dai J, Jiang L, Qiu L, Shao Y, Shi P, Li J
OncoTargets and therapy 2020;13:7033-7044

Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α.

Li H, Ma Z, Zhai Y, Lv C, Yuan P, Zhu F, Wei L, Li Q, Qi X
Frontiers in pharmacology 2020;11:1255

Pimozide Alleviates Hyperglycemia in Diet-Induced Obesity by Inhibiting Skeletal Muscle Ketone Oxidation.

Al Batran R, Gopal K, Capozzi ME, Chahade JJ, Saleme B, Tabatabaei-Dakhili SA, Greenwell AA, Niu J, Almutairi M, Byrne NJ, Masson G, Kim R, Eaton F, Mulvihill EE, Garneau L, Masters AR, Desta Z, Velázquez-Martínez CA, Aguer C, Crawford PA, Sutendra G, Campbell JE, Dyck JRB, Ussher JR
Cell metabolism 2020 May 5;31(5):909-919.e8

β-Hydroxybutyrate Increases Exercise Capacity Associated with Changes in Mitochondrial Function in Skeletal Muscle.

Monsalves-Alvarez M, Morales PE, Castro-Sepulveda M, Sepulveda C, Rodriguez JM, Chiong M, Eisner V, Lavandero S, Troncoso R
Nutrients 2020 Jun 29;12(7)

Effects of a Ketogenic Diet Containing Medium-Chain Triglycerides and Endurance Training on Metabolic Enzyme Adaptations in Rat Skeletal Muscle.

Fukazawa A, Koike A, Karasawa T, Tsutsui M, Kondo S, Terada S
Nutrients 2020 Apr 30;12(5)

Ketogenic diet activates protective γδ T cell responses against influenza virus infection.

Goldberg EL, Molony RD, Kudo E, Sidorov S, Kong Y, Dixit VD, Iwasaki A
Science immunology 2019 Nov 15;4(41)

Role of OXCT1 in ovine adipose and preadipocyte differentiation.

Zeng J, Zhou SW, Zhao J, Jin MH, Kang DJ, Yang YX, Wang XL, Chen YL
Biochemical and biophysical research communications 2019 May 14;512(4):779-785

Ketogenic diet ameliorates axonal defects and promotes myelination in Pelizaeus-Merzbacher disease.

Stumpf SK, Berghoff SA, Trevisiol A, Spieth L, Düking T, Schneider LV, Schlaphoff L, Dreha-Kulaczewski S, Bley A, Burfeind D, Kusch K, Mitkovski M, Ruhwedel T, Guder P, Röhse H, Denecke J, Gärtner J, Möbius W, Nave KA, Saher G
Acta neuropathologica 2019 Jul;138(1):147-161

Role and therapeutic potential of dietary ketone bodies in lymph vessel growth.

García-Caballero M, Zecchin A, Souffreau J, Truong AK, Teuwen LA, Vermaelen W, Martín-Pérez R, de Zeeuw P, Bouché A, Vinckier S, Cornelissen I, Eelen G, Ghesquière B, Mazzone M, Dewerchin M, Carmeliet P
Nature metabolism 2019 Jul;1(7):666-675

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Puchalska P, Martin SE, Huang X, Lengfeld JE, Daniel B, Graham MJ, Han X, Nagy L, Patti GJ, Crawford PA
Cell metabolism 2019 Feb 5;29(2):383-398.e7

Cancer stem-like properties and gefitinib resistance are dependent on purine synthetic metabolism mediated by the mitochondrial enzyme MTHFD2.

Nishimura T, Nakata A, Chen X, Nishi K, Meguro-Horike M, Sasaki S, Kita K, Horike SI, Saitoh K, Kato K, Igarashi K, Murayama T, Kohno S, Takahashi C, Mukaida N, Yano S, Soga T, Tojo A, Gotoh N
Oncogene 2019 Apr;38(14):2464-2481

Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.

Zhang J, Jia PP, Liu QL, Cong MH, Gao Y, Shi HP, Yu WN, Miao MY
Journal of lipid research 2018 Apr;59(4):625-634

E2F6 Impairs Glycolysis and Activates BDH1 Expression Prior to Dilated Cardiomyopathy.

Major JL, Dewan A, Salih M, Leddy JJ, Tuana BS
PloS one 2017;12(1):e0170066

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.

Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology 2017 Mar 6;216(3):675-693

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Huang D, Li T, Wang L, Zhang L, Yan R, Li K, Xing S, Wu G, Hu L, Jia W, Lin SC, Dang CV, Song L, Gao P, Zhang H
Cell research 2016 Oct;26(10):1112-1130

The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.

Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD
Nature medicine 2015 Mar;21(3):263-9

Impact of peripheral ketolytic deficiency on hepatic ketogenesis and gluconeogenesis during the transition to birth.

Cotter DG, Ercal B, d'Avignon DA, Dietzen DJ, Crawford PA
The Journal of biological chemistry 2013 Jul 5;288(27):19739-49

Successful adaptation to ketosis by mice with tissue-specific deficiency of ketone body oxidation.

Cotter DG, Schugar RC, Wentz AE, d'Avignon DA, Crawford PA
American journal of physiology. Endocrinology and metabolism 2013 Feb 15;304(4):E363-74

Obligate role for ketone body oxidation in neonatal metabolic homeostasis.

Cotter DG, d'Avignon DA, Wentz AE, Weber ML, Crawford PA
The Journal of biological chemistry 2011 Mar 4;286(9):6902-10

Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy.

Maurer GD, Brucker DP, Bähr O, Harter PN, Hattingen E, Walenta S, Mueller-Klieser W, Steinbach JP, Rieger J
BMC cancer 2011 Jul 26;11:315

Adaptation of myocardial substrate metabolism to a ketogenic nutrient environment.

Wentz AE, d'Avignon DA, Weber ML, Cotter DG, Doherty JM, Kerns R, Nagarajan R, Reddy N, Sambandam N, Crawford PA
The Journal of biological chemistry 2010 Aug 6;285(32):24447-56

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human brain tissue were subjected to SDS PAGE followed by western blot with 12175-1-AP(SCOT antibody) at dilution of 1:400
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line