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- References [20]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 14112-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- RNF8 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RNF8 antibody (Cat. #14112-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references SiRNF8 Delivered by DNA Framework Nucleic Acid Effectively Sensitizes Chemotherapy in Colon Cancer.
RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.
CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma.
SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas.
Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3β Inhibition.
53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.
Protective Effects of Sesamin on Cytoxan-Induced Spermatogenesis Dysfunction by Regulating RNF8-ubH2A/ubH2B Pathways in Male Mice.
Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.
RNF8 is not required for histone-to-protamine exchange in spermiogenesis†.
RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.
Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.
RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.
UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.
BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.
The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
L3MBTL2 regulates chromatin remodeling during spermatogenesis.
The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors.
PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.
GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.
Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.
Guo Z, Song H, Tian Y, Xu J, Zhang G, Guo Y, Shen R, Wang D
International journal of nanomedicine 2024;19:171-188
International journal of nanomedicine 2024;19:171-188
RNF8 enhances the sensitivity of PD-L1 inhibitor against melanoma through ubiquitination of galectin-3 in stroma.
Guo Y, Shen R, Yang K, Wang Y, Song H, Liu X, Cheng X, Wu R, Song Y, Wang D
Cell death discovery 2023 Jun 30;9(1):205
Cell death discovery 2023 Jun 30;9(1):205
CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma.
Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST
Cell death and differentiation 2023 Aug;30(8):1973-1987
Cell death and differentiation 2023 Aug;30(8):1973-1987
SET8 Inhibition Potentiates Radiotherapy by Suppressing DNA Damage Repair in Carcinomas.
Pan D, Du YR, Li R, Shen AH, Liu XD, Li CY, Hu BR
Biomedical and environmental sciences : BES 2022 Mar 20;35(3):194-205
Biomedical and environmental sciences : BES 2022 Mar 20;35(3):194-205
Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3β Inhibition.
Zhu X, Li J, You D, Xiao Y, Huang Z, Yu W
Molecular neurobiology 2022 Aug;59(8):4776-4790
Molecular neurobiology 2022 Aug;59(8):4776-4790
53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling.
Tsaridou S, Velimezi G, Willenbrock F, Chatzifrangkeskou M, Elsayed W, Panagopoulos A, Karamitros D, Gorgoulis V, Lygerou Z, Roukos V, O'Neill E, Pefani DE
EMBO reports 2022 Aug 3;23(8):e54483
EMBO reports 2022 Aug 3;23(8):e54483
Protective Effects of Sesamin on Cytoxan-Induced Spermatogenesis Dysfunction by Regulating RNF8-ubH2A/ubH2B Pathways in Male Mice.
Hai DM, Ren JW, Chi YN, Ye RJ, Liu N, Ma L, Lan XB, Wu J, Yu JQ, Yang JM
Frontiers in pharmacology 2021;12:708467
Frontiers in pharmacology 2021;12:708467
Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.
Liu J, Li X, Zhou G, Zhang Y, Sang Y, Wang J, Li Y, Ge W, Sun Z, Zhou X
Environmental pollution (Barking, Essex : 1987) 2021 Sep 1;284:117446
Environmental pollution (Barking, Essex : 1987) 2021 Sep 1;284:117446
RNF8 is not required for histone-to-protamine exchange in spermiogenesis†.
Abe H, Meduri R, Li Z, Andreassen PR, Namekawa SH
Biology of reproduction 2021 Nov 15;105(5):1154-1159
Biology of reproduction 2021 Nov 15;105(5):1154-1159
RNF8 ubiquitinates RecQL4 and promotes its dissociation from DNA double strand breaks.
Tan Q, Niu K, Zhu Y, Chen Z, Li Y, Li M, Wei D, Balajee AS, Fang H, Zhao Y
Oncogenesis 2021 Mar 5;10(3):24
Oncogenesis 2021 Mar 5;10(3):24
Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.
Hang Q, Zeng L, Wang L, Nie L, Yao F, Teng H, Deng Y, Yap S, Sun Y, Frank SJ, Chen J, Ma L
Nature communications 2021 Jun 29;12(1):4033
Nature communications 2021 Jun 29;12(1):4033
RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.
Lu X, Xu M, Zhu Q, Zhang J, Liu G, Bao Y, Gu L, Tian Y, Wen H, Zhu WG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21326
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Apr;35(4):e21326
UBE2T-regulated H2AX monoubiquitination induces hepatocellular carcinoma radioresistance by facilitating CHK1 activation.
Sun J, Zhu Z, Li W, Shen M, Cao C, Sun Q, Guo Z, Liu L, Wu D
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222
Journal of experimental & clinical cancer research : CR 2020 Oct 21;39(1):222
BGL3 lncRNA mediates retention of the BRCA1/BARD1 complex at DNA damage sites.
Hu Z, Mi S, Zhao T, Peng C, Peng Y, Chen L, Zhu W, Yao Y, Song Q, Li X, Li X, Jia C, Pei H
The EMBO journal 2020 Jun 17;39(12):e104133
The EMBO journal 2020 Jun 17;39(12):e104133
The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.
Singh AN, Oehler J, Torrecilla I, Kilgas S, Li S, Vaz B, Guérillon C, Fielden J, Hernandez-Carralero E, Cabrera E, Tullis ID, Meerang M, Barber PR, Freire R, Parsons J, Vojnovic B, Kiltie AE, Mailand N, Ramadan K
The EMBO journal 2019 Oct 4;38(21):e102361
The EMBO journal 2019 Oct 4;38(21):e102361
L3MBTL2 regulates chromatin remodeling during spermatogenesis.
Meng C, Liao J, Zhao D, Huang H, Qin J, Lee TL, Chen D, Chan WY, Xia Y
Cell death and differentiation 2019 Nov;26(11):2194-2207
Cell death and differentiation 2019 Nov;26(11):2194-2207
The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors.
Peng Y, Liao Q, Tan W, Peng C, Hu Z, Chen Y, Li Z, Li J, Zhen B, Zhu W, Li X, Yao Y, Song Q, Liu C, Qi X, He F, Pei H
Nature communications 2019 Mar 15;10(1):1224
Nature communications 2019 Mar 15;10(1):1224
PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.
Wang X, Kang JY, Wei L, Yang X, Sun H, Yang S, Lu L, Yan M, Bai M, Chen Y, Long J, Li N, Li D, Huang J, Lei M, Shao Z, Yuan W, Zuo E, Lu K, Liu MF, Li J
Development (Cambridge, England) 2019 Jul 10;146(13)
Development (Cambridge, England) 2019 Jul 10;146(13)
GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.
Lu X, Tang M, Zhu Q, Yang Q, Li Z, Bao Y, Liu G, Hou T, Lv Y, Zhao Y, Wang H, Yang Y, Cheng Z, Wen H, Liu B, Xu X, Gu L, Zhu WG
Nucleic acids research 2019 Dec 2;47(21):10977-10993
Nucleic acids research 2019 Dec 2;47(21):10977-10993
Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.
Gou LT, Kang JY, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua MM, Lu Y, Zhu Y, Li Z, Chen H, Wu LG, Li D, Fu XD, Li J, Shi HJ, Liu MF
Cell 2017 Jun 1;169(6):1090-1104.e13
Cell 2017 Jun 1;169(6):1090-1104.e13
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP( RNF8 Antibody) at dilution of 1:600
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RNF8 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RNF8. This antibody recognizes human,mouse,rat antigen. The RNF8 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
