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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- LS-C313004 - Provider product page
- Provider
- LSBio
- Product name
- SF1 Antibody (aa11-30) LS-C313004
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- WB of SF1 antibody. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: PANC Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SW620 Cell Lysate. Lane 6: SKOV Cell Lysate.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-splicing factor 1 antibody, Western blotting All lanes: Anti splicing factor 1 at 0.5ug/ml Lane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KD Observed bind size: 70KD
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SF1 antibody. ICC: HELA Cell.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- SF1 antibody. ICC: HELA Cell.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SF1 antibody. IHC(F): Rat Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SF1 antibody. IHC(P): Human Lung Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-splicing factor 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SF1 antibody. IHC(P): Human Lung Cancer Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- SF1 antibody. IHC(F): Rat Spleen Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-splicing factor 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
