Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4176 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CaM Kinase II delta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CaM Kinase II delta isoforms. No cross-reactivity with recombinant human CaM Kinase II alpha , CaM Kinase II beta , or CaM Kinase II gamma is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q13557
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CaM Kinase II delta by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase II delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase II delta at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CaM Kinase II delta in Human Brain. CaM Kinase II delta was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase II delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.