Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB4176 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CaM Kinase II delta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human CaM Kinase II delta isoforms in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKII alpha , CaMKII beta , or CaMKII gamma .
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13557
- Isotype
- IgG
- Antibody clone number
- 438422
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Deletion of Dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission.
MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels.
Sadegh MK, Ekman M, Rippe C, Uvelius B, Swärd K, Albinsson S
PloS one 2012;7(4):e35882
PloS one 2012;7(4):e35882
MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels.
Turczynska KM, Sadegh MK, Hellstrand P, Swärd K, Albinsson S
The Journal of biological chemistry 2012 Jun 1;287(23):19199-206
The Journal of biological chemistry 2012 Jun 1;287(23):19199-206
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human CaM Kinase II delta by Western Blot. Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase II delta Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase II delta isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.