PA5-54670

antibody from Invitrogen Antibodies

Targeting: SLFN11 FLJ34922

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54670

Provider

Invitrogen Antibodies

Product name

SLFN11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: REVLGCAKEN VDPDSLRRKI EQAIYKLPCV HFCQPQRPIT FTLKIVDVLK RGELYGYACM IRVNPFCCAV FSEAPNSWI Highest antigen sequence identity to the following orthologs: Mouse - 52%, Rat - 53%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLFN11 in human cell line HEK 293 and human cell line MCF-7 using a SLFN11 Polyclonal Antibody (Product # PA5-54670).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLFN11 in human cell line MOLT-4 using a SLFN11 Polyclonal Antibody (Product # PA5-54670).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of SLFN11 in human cell line A-431 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a SLFN11 Polyclonal Antibody (Product # PA5-54670).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of SLFN11 in human cell line A-431 using a SLFN11 Polyclonal Antibody (Product # PA5-54670) shows localization to nucleoplasm and cytosol.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SLFN11 in human fallopian tube and skeletal muscle tissues using SLFN11 Polyclonal Antibody (Product # PA5-54670). Corresponding SLFN11 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SLFN11 in human fallopian tube using SLFN11 Polyclonal Antibody (Product # PA5-54670) shows moderate to strong nuclear positivity in glandular cells, as well as in lymphoid cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SLFN11 in human lymphoid tissues using SLFN11 Polyclonal Antibody (Product # PA5-54670) shows moderate to strong nuclear positivity in lymphoid cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SLFN11 in human stomach cancer using SLFN11 Polyclonal Antibody (Product # PA5-54670) shows moderate to strong nuclear positivity in tumor cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of SLFN11 in human skeletal muscle using SLFN11 Polyclonal Antibody (Product # PA5-54670) shows no positivity in myocytes as expected.