Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-92368 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-92368, RRID:AB_11027208
- Product name
- Rabbit Polyclonal Schlafen 11 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Schlafen 11 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11.
Li M, Kao E, Gao X, Sandig H, Limmer K, Pavon-Eternod M, Jones TE, Landry S, Pan T, Weitzman MD, David M
Nature 2012 Nov 1;491(7422):125-8
Nature 2012 Nov 1;491(7422):125-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Schlafen 11 Antibody [NBP1-92368] - Simple Western lane view shows a specific band for SLFN11 in 0.2 mg/ml of MOLT-4 (left), HEK 239T (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Schlafen 11 Antibody [NBP1-92368] - Electropherogram image(s) of corresponding Simple Western lane view. Schlafen 8 antibody was used at 1:20 dilution on MOLT-4 and HEK 239T lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Schlafen 11 Antibody [NBP1-92368] - Analysis in human cell line MOLT-4.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Schlafen 11 Antibody [NBP1-92368] - Analysis in human cell lines HEK293 and Caco-2 using anti-SLFN11 antibody. Corresponding SLFN11 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Schlafen 11 Antibody [NBP1-92368] - Immunohistochemistry analysis in human fallopian tube and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Schlafen 11 Antibody [NBP1-92368] - Staining of human lymphoid tissues shows moderate to strong nuclear positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Schlafen 11 Antibody [NBP1-92368] - Staining of human stomach cancer shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Schlafen 11 Antibody [NBP1-92368] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Schlafen 11 Antibody [NBP1-92368] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells, as well as in lymphoid cells.