TA501082

antibody from OriGene

Targeting: LIPG EDL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501082

Provider

OriGene

Proper citation

OriGene Cat#TA501082, RRID:AB_11124365

Product name

LIPG mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Antibody type

Monoclonal

Description

LIPG mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Host

Mouse

Conjugate

Unconjugated

Epitope

LIPG

Isotype

IgG

Antibody clone number

OTI1E3

Vial size

100 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIPG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-LIPG mouse monoclonal antibody (TA501082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG(RC209248).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC209248 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIPG antibody(TA501082), and then analyzed by flow cytometry.

Validation comment

FC