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ELISAAntibody data
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- Antigen structure
- References [2]
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- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-16799 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIPG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody does not react with mouse samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references A novel NanoBiT-based assay monitors the interaction between lipoprotein lipase and GPIHBP1 in real time.
The effect of high glucose on lipid metabolism in the human placenta.
Shetty SK, Walzem RL, Davies BSJ
Journal of lipid research 2020 Apr;61(4):546-559
Journal of lipid research 2020 Apr;61(4):546-559
The effect of high glucose on lipid metabolism in the human placenta.
Hulme CH, Nicolaou A, Murphy SA, Heazell AEP, Myers JE, Westwood M
Scientific reports 2019 Oct 1;9(1):14114
Scientific reports 2019 Oct 1;9(1):14114
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human endothelial lipase detected in transfected 293 lysates using Product # PA1-16799. (MW 57 kD)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelial Lipase in ECV304 cells using Product # PA1-16799.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LIPG in transfected 293 lysates. Sample was incubated in LIPG polyclonal antibody (Product # PA1-16799).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LIPG in ECV304 cells. Sample was incubated in LIPG polyclonal antibody (Product # PA1-16799).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LIPG in HeLa cells. Samples were incubated in LIPG polyclonal antibody (Product # PA1-16799) followed by DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Placental tissue from pregnancies complicated by T1DM (n = 6) or T2DM (n = 6) and uncomplicated term pregnancies (maternal BMI < 30 (n = 6) and >30 (n = 6)) were stained using antibodies that recognise (A ) endothelial lipase, (B) lipoprotein lipase, (C) fatty acid translocase, (D) fatty acid transporter protein (FATP) 2 or (E) FATP4 then analysed by scoring the intensity of staining in the trophoblast and endothelium as low (0), medium (1) or high (2). Data points represent the mean score from ten images, with the median value plotted as a bar. Circle - T1DM; square - T2DM; triangle - control BMI < 30; inverted triangle - control BMI > 30. Data were compared using the Kruskal-Wallis test; no differences in staining for the lipases or fatty acid transporters were observed, in either compartment, between the subject groups (p > 0.05).
