PA5-31115

antibody from Invitrogen Antibodies

Targeting: KLHL25 ENC-2, ENC2, FLJ12587

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31115

Provider

Invitrogen Antibodies

Product name

ENC-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: NT2D1.

Concentration

0.69 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENC-2 Polyclonal Antibody (Product # PA5-31115). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 7.5% SDS PAGE. ENC-2 Polyclonal Antibody (Product # PA5-31115) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of KLHL25 in methanol-fixed MCF-7 cells using a KLHL25 polyclonal antibody (Product # PA5-31115) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KLHL25 (Product # PA5-31115) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse muscle, using KLHL25 (Product # PA5-31115) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENC-2 Polyclonal Antibody detects KLHL25 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. ENC-2 Polyclonal Antibody (Product # PA5-31115) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENC-2 Polyclonal Antibody detects KLHL25 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. ENC-2 Polyclonal Antibody (Product # PA5-31115) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.