Explore
Validate
LearnA301-954A
antibody from Invitrogen Antibodies
Targeting: PARP4
ADPRTL1, ARTD4, p193, VAULT3, VPARP, VWA5C
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A301-954A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- vPARP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human vPARP by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-vPARP antibody A301-954A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody A301-953A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human vPARP by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- vPARP (Cat. No. A301-954A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
