Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 101-M28 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-1/Flt-1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human soluble Flt-1(D1-5)
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKW
SLPEMVSKESERLSITKSACGRNGKQFCSTLTLNT
AQANHTGFYSCKYLAVPTSKKKETESAIYIFISDT
GRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNIT
VTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKE
IGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQIST
PRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDE
KNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDK
GLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQ
QVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPA
TEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQ
SNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPL
GSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCD
FCSNNEESFILDADSNMGNRIESITQRMAIIEGKN
KMASTLVVADSRISGIYICIASNKVGTVGRNISFY
ITDVPNGFHVN- Isotype
- IgG
- Antibody clone number
- (#EWF)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references VEGF-A and PlGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells
Fiedler J, Leucht F, Waltenberger J, Dehio C, Brenner R
Biochemical and Biophysical Research Communications 2005 August;334(2):561-568
Biochemical and Biophysical Research Communications 2005 August;334(2):561-568
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western analysis with anti-human VEGFR-1 (#EWF) using rh sFlt-1(D4) conditioned supernatant derived from insect cells.
- Sample type
- Sf9 conditioned media