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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 102-PA21S - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-1/Flt-1 (Peptide)
- Antibody type
- Polyclonal
- Antigen
- Peptide: GEHCNKKAVFSRISKFKSTR NDSTTQSNVKH.
- Description
- antibody Protein-A purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
GEHCNKKAVFSRISKFKSTR NDSTTQSNVKH.- Antibody clone number
- Rabbit IG
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human esFlt-1. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions. Left panel: monoclonal antibody against Flt-1; Right panel: polyclonal antibody (peptide) against the unique C-terminal end of esFlt-1.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of two neighboring sections of a human vein (V), located near a hemangioma. The antibody against the soluble VEGFR-1/Flt-1 marked single cells (arrows) within the media and adventitia of the vein. The antibody against the membrane-bound VEGFR-1/Flt-1 marked single cells (arrows) and the endothelium (arrowhead) of the vein. Cell nuclei are stained with Dapi (blue). Provided by Prof. J. Wilting, Göttingen, Germany.
