Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF4170 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal VEGFR1/Flt-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VEGFR1/Flt-1 when phosphorylated at Y1213.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Upregulation of RASSF1A in Colon Cancer by Suppression of Angiogenesis Signaling and Akt Activation.
Treatment with placental growth factor attenuates myocardial ischemia/reperfusion injury.
Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion.
The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells.
Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1α-VEGF pathway inhibition.
BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells.
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
Blanchard TG, Lapidus R, Banerjee V, Bafford AC, Czinn SJ, Ahmed H, Banerjee A
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(3):1259-1273
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;48(3):1259-1273
Treatment with placental growth factor attenuates myocardial ischemia/reperfusion injury.
Zhang Y, Cao C, Xin J, Lv P, Chen D, Li S, Yang H, Chen C, Liu B, Li Q
PloS one 2018;13(9):e0202772
PloS one 2018;13(9):e0202772
Intracrine VEGF signalling mediates colorectal cancer cell migration and invasion.
Bhattacharya R, Fan F, Wang R, Ye X, Xia L, Boulbes D, Ellis LM
British journal of cancer 2017 Sep 5;117(6):848-855
British journal of cancer 2017 Sep 5;117(6):848-855
The anti-vascular endothelial growth factor receptor-1 monoclonal antibody D16F7 inhibits invasiveness of human glioblastoma and glioblastoma stem cells.
Atzori MG, Tentori L, Ruffini F, Ceci C, Lisi L, Bonanno E, Scimeca M, Eskilsson E, Daubon T, Miletic H, Ricci Vitiani L, Pallini R, Navarra P, Bjerkvig R, D'Atri S, Lacal PM, Graziani G
Journal of experimental & clinical cancer research : CR 2017 Aug 10;36(1):106
Journal of experimental & clinical cancer research : CR 2017 Aug 10;36(1):106
Deletion of placental growth factor prevents diabetic retinopathy and is associated with Akt activation and HIF1α-VEGF pathway inhibition.
Huang H, He J, Johnson D, Wei Y, Liu Y, Wang S, Lutty GA, Duh EJ, Semba RD
Diabetes 2015 Jan;64(1):200-12
Diabetes 2015 Jan;64(1):200-12
BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi RK, Xu W, Karakousis G, Gangadhar TC, Schuchter LM, Lieu M, Khare S, Halloran MB, Herlyn M, Kaufman RE
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Apr 1;21(7):1652-64
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Apr 1;21(7):1652-64
Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells.
Attar-Schneider O, Drucker L, Zismanov V, Tartakover-Matalon S, Rashid G, Lishner M
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):178-90
Laboratory investigation; a journal of technical methods and pathology 2012 Feb;92(2):178-90
Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
Meda C, Molla F, De Pizzol M, Regano D, Maione F, Capano S, Locati M, Mantovani A, Latini R, Bussolino F, Giraudo E
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4081-92
Journal of immunology (Baltimore, Md. : 1950) 2012 Apr 15;188(8):4081-92
Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
The Journal of biological chemistry 2008 Dec 5;283(49):34250-9
The Journal of biological chemistry 2008 Dec 5;283(49):34250-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-VEGF R1/Flt-1 (Y1213) by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-VEGF R1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGF R1/Flt-1 (Y1213) at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
