Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- FAB321P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal VEGFR1/Flt-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references A quantitative method for screening and identifying molecular targets for nanomedicine.
The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia.
BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
Metabolic impact of anti-angiogenic agents on U87 glioma cells.
Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
Laminin promotes vascular network formation in 3D in vitro collagen scaffolds by regulating VEGF uptake.
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation.
Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
Expansion of human SCID-repopulating cells under hypoxic conditions.
Guo P, Yang J, Bielenberg DR, Dillon D, Zurakowski D, Moses MA, Auguste DT
Journal of controlled release : official journal of the Controlled Release Society 2017 Oct 10;263:57-67
Journal of controlled release : official journal of the Controlled Release Society 2017 Oct 10;263:57-67
The distribution and potential prognostic value of SMAD protein expression in chronic lymphocytic leukemia.
Witkowska M, Majchrzak A, Cebula-Obrzut B, Wawrzyniak E, Robak T, Smolewski P
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Mar;39(3):1010428317694551
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 Mar;39(3):1010428317694551
BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi RK, Xu W, Karakousis G, Gangadhar TC, Schuchter LM, Lieu M, Khare S, Halloran MB, Herlyn M, Kaufman RE
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Apr 1;21(7):1652-64
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Apr 1;21(7):1652-64
Metabolic impact of anti-angiogenic agents on U87 glioma cells.
Mesti T, Savarin P, Triba MN, Le Moyec L, Ocvirk J, Banissi C, Carpentier AF
PloS one 2014;9(6):e99198
PloS one 2014;9(6):e99198
Antiangiogenic cancer drug sunitinib exhibits unexpected proangiogenic effects on endothelial cells.
Norton KA, Han Z, Popel AS, Pandey NB
OncoTargets and therapy 2014;7:1571-82
OncoTargets and therapy 2014;7:1571-82
Laminin promotes vascular network formation in 3D in vitro collagen scaffolds by regulating VEGF uptake.
Stamati K, Priestley JV, Mudera V, Cheema U
Experimental cell research 2014 Sep 10;327(1):68-77
Experimental cell research 2014 Sep 10;327(1):68-77
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
Fiorio Pla A, Brossa A, Bernardini M, Genova T, Grolez G, Villers A, Leroy X, Prevarskaya N, Gkika D, Bussolati B
BMC cancer 2014 Dec 12;14:939
BMC cancer 2014 Dec 12;14:939
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
Park SJ, Kim KJ, Kim WU, Cho CS
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
Journal of thrombosis and haemostasis : JTH 2011 May;9(5):1056-66
Journal of thrombosis and haemostasis : JTH 2011 May;9(5):1056-66
Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis.
Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G
Blood 2010 Oct 21;116(16):3099-107
Blood 2010 Oct 21;116(16):3099-107
Multiple receptor tyrosine kinases regulate HIF-1alpha and HIF-2alpha in normoxia and hypoxia in neuroblastoma: implications for antiangiogenic mechanisms of multikinase inhibitors.
Nilsson MB, Zage PE, Zeng L, Xu L, Cascone T, Wu HK, Saigal B, Zweidler-McKay PA, Heymach JV
Oncogene 2010 May 20;29(20):2938-49
Oncogene 2010 May 20;29(20):2938-49
VEGFR2 is selectively expressed by FOXP3high CD4+ Treg.
Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M
European journal of immunology 2010 Jan;40(1):197-203
European journal of immunology 2010 Jan;40(1):197-203
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Makino H, Ranjan P, Minakuchi K, Sone S
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A
Journal of cellular physiology 2008 Oct;217(1):261-80
Journal of cellular physiology 2008 Oct;217(1):261-80
Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation.
Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N
Experimental hematology 2008 May;36(5):545-558
Experimental hematology 2008 May;36(5):545-558
Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
Narazaki M, Segarra M, Tosato G
Blood 2008 Apr 15;111(8):4126-36
Blood 2008 Apr 15;111(8):4126-36
Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
The Journal of clinical investigation 2007 Nov;117(11):3369-82
The Journal of clinical investigation 2007 Nov;117(11):3369-82
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Ball SG, Shuttleworth CA, Kielty CM
The Journal of cell biology 2007 May 7;177(3):489-500
The Journal of cell biology 2007 May 7;177(3):489-500
Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
Karrar A, Broomé U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
Gut 2007 Feb;56(2):243-52
Gut 2007 Feb;56(2):243-52
Expansion of human SCID-repopulating cells under hypoxic conditions.
Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
The Journal of clinical investigation 2003 Jul;112(1):126-35
The Journal of clinical investigation 2003 Jul;112(1):126-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of VEGF R1/Flt-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R1/Flt-1 PE-conjugated Monoclonal Antibody (Catalog # FAB321P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.