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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB321-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGFR1/Flt-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGFR1, recombinant human (rh) VEGFR2, rhVEGFR3, or rhVEGFR4.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_001153392- Isotype
- IgG
- Antibody clone number
- 49560
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells.
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Odent Grigorescu G, Rosca AM, Preda MB, Tutuianu R, Simionescu M, Burlacu A
Journal of tissue engineering and regenerative medicine 2017 Nov;11(11):3241-3252
Journal of tissue engineering and regenerative medicine 2017 Nov;11(11):3241-3252
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
Jarajapu YP, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter SK, Raizada MK, Boulton ME, Grant MB
PloS one 2014;9(4):e93965
PloS one 2014;9(4):e93965
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30
Nature 2011 Jul 13;475(7355):226-30
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis 2010 Dec;13(4):337-47
Angiogenesis 2010 Dec;13(4):337-47
Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J
Cancer research 2009 Mar 1;69(5):1976-84
Cancer research 2009 Mar 1;69(5):1976-84
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foà R, Peschle C, Sporn M, Testa U
Leukemia research 2008 Aug;32(8):1244-58
Leukemia research 2008 Aug;32(8):1244-58
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Caldas H, Fangusaro JR, Boué DR, Holloway MP, Altura RA
Blood 2007 Feb 15;109(4):1479-89
Blood 2007 Feb 15;109(4):1479-89
Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Lin YL, Liang YC, Chiang BL
Journal of leukocyte biology 2007 Dec;82(6):1473-80
Journal of leukocyte biology 2007 Dec;82(6):1473-80
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foà R, Peschle C, Testa U
Stem cells (Dayton, Ohio) 2007 Aug;25(8):1862-71
Stem cells (Dayton, Ohio) 2007 Aug;25(8):1862-71
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VEGFR1/Flt-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells was stained with Mouse Anti-Human VEGFR1/Flt-1 Monoclonal Antibody (Catalog # MAB321, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
