H00002321-D01P

antibody from Abnova Corporation

Targeting: FLT1 FLT, VEGFR1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002321-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002321-D01P, RRID:AB_1573603

Product name

FLT1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human FLT1 protein.

Antigen sequence

MVSYWDTGVLLCALLSCLLLTGSSSGSKLKDPELS
LKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKE
SERLSITKSACGRNGKQFCSTLTLNTAQANHTGFY
SCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYS
EIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLD
TLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEAT
VNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRG
HTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRR
RIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRS
GPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGK
RSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLT
RGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTA
TLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCT
AYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESF
ILDADSNMGNRIESITQRMAIIEGKNKMASTLVVA
DSRISGIYICIASNKVGTVGRNISFYITDVPNGFH
VNLEKMPTEGEDLKLSCTVNKFLYRDVTWILLRTV
NNRTMHYSISKQKMAITKEHSITLNLTIMNVSLQD
SGTYACRARNVYTGEEILQKKEITIRGEHCNKKAV
FSRISKFKSTRNDCTTQSNVKH


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FLT1 expression in transfected 293T cell line (H00002321-T01) by FLT1 MaxPab polyclonal antibody.Lane 1: FLT1 transfected lysate(77.50 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FLT1 and CRKL. HeLa cells were stained with anti-FLT1 rabbit purified polyclonal 1:100 and anti-CRKL mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)