Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00534 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-VEGF Receptor 1/FLT1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for VEGFR1/FLT1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. VEGFR1/FLT1 information: Subcellular Localization: Isoform 1: Cell membrane; Autophosphorylation promotes ubiquitination and endocytosis; Tissue Specificity: Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway.
Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory Cytokines and the VEGF-PI3K/Akt-eNOS Signaling Pathway.
The impact of vascular endothelial growth factor-transfected human endothelial cells on endothelialization and restenosis of stainless steel stents.
Yang KJ, Choi WJ, Chang YK, Park CW, Kim SY, Hong YA
International journal of molecular sciences 2023 Feb 14;24(4)
International journal of molecular sciences 2023 Feb 14;24(4)
Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory Cytokines and the VEGF-PI3K/Akt-eNOS Signaling Pathway.
Xu Z, Zhang X, Lu R, Zhang D, Zou T, Chen M, Zhang D
Evidence-based complementary and alternative medicine : eCAM 2022;2022:4621131
Evidence-based complementary and alternative medicine : eCAM 2022;2022:4621131
The impact of vascular endothelial growth factor-transfected human endothelial cells on endothelialization and restenosis of stainless steel stents.
Tang C, Wang G, Wu X, Li Z, Shen Y, Lee JC, Yu Q
Journal of vascular surgery 2011 Feb;53(2):461-71
Journal of vascular surgery 2011 Feb;53(2):461-71
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human A549 cell lysate,Lane 4: human COLO-320 cell lysate,Lane 5: Human A431 cell lysate,Lane 6: human SK-OV-3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 125KD. The expected band size for VEGF Receptor 1 is at 150KD.
- Additional image