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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
- Functional assay [1]
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- Product number
- 101-M29 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- Anti-human VEGFR1-14/sFlt1-14
- Antibody type
- Monoclonal
- Description
- antibody Protein-G purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
DQEAPYLLRNLSDH- Antibody clone number
- (#180F3B4)
- Storage
- The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
- Handling
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Blot Analysis of recombinant human sFlt-1(D5), sFlt-1, sFlt1-14, sKDR(D7) and sFLT-4 using a monoclonal mouse anti-human sFlt1-14 antibody generated against a peptide from the unique C terminal end (left panel) and a monoclonal mouse anti-human FLT-1 recognizing all Flt-1 proteins (right panel).
- Sample type
- purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Flt-1 Sandwich-ELISA using recombinant human sFlt1-14 as standard. Mouse anti-human Flt-1 #EWI [Cat# 101-M30] was used as capture antibody, Biotinylated rabbit anti-human Flt-1 [Cat# 102-PABi20] was used for detection.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of cryo-sections of human foreskin with mouse anti-human sFlt1-14 antibody and counter staining of nuclei with Dapi (blue). (B) Control; Fixation: 4% PFA (app. 25min); Dilution: 1:500. Staining in smooth muscle cells, the perineurium of nerves and the Stratum basale of the epidermis as well as some scattered cells. The experiment was performed by the research group of Prof. Dr. J. Wilting and M. Lohrberg, University Göttingen, Germany.
- Sample type
- human foreskin
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunoperoxidase staining of paraffin-embedded sections of human foreskin with mouse anti-human sFlt1-14 antibody. (D) Control; Fixation: 4% PFA (app. 25min); Dilution: 1:100-500. The staining in smooth muscle cells is visible. The experiment was performed by the research group of Prof. Dr. J. Wilting and M. Lohrberg, University Göttingen, Germany.
- Sample type
- human foreskin
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Direct ELISA: recombinant human sFlt-1(D5), sFlt-1 and sFlt1-14 were coated with 1µg/ml in PBS. The sFlt1-14 specific mouse anti-human sFlt1-14 antibody generated against a peptide from the unique C terminal end [Cat# 101-M29] was used with 2µg/ml, the Flt-1 specific mouse anti-human Flt-1 #EWI [Cat# 101-M30] recognizing all Flt-1 proteins was used as control [1µg/ml].
- Sample type
- purified recombinant proteins
