Ab176419

antibody from Aladdin Scientific

Targeting: FLT1 FLT, VEGFR1

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab176419

Provider

Aladdin Scientific

Product name

sFlt-1 Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human sFlt-1 Antibody, Monoclonal (2A6-4), is a Detection or Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA.CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AEFLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

2A6-4

Vial size

100_g,10_g,1mg,50_g

Concentration

Lot by Lot

Storage

Store at 37¡C short term_7 days_Store at 4¡C short term_30 days_. Store at -20±5¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

ELISA

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Sandwich ELISA - sFlt-1 protein standard curve Human sFlt-1 protein was serially diluted 2-fold and captured by sFlt-1 Mouse mAb (Ab176421) coated on a Clear Polystyrene Microplate (W291037). sFlt-1 Mouse mAb (Ab176419) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Binding ELISA - sFlt-1 Mouse mAb (Ab176419) Immobilized Recombinant Human sFlt-1 Protein at 1.0 μg/mL can bind sFlt-1 Mouse mAb (Ab176419) with the EC₅₀ of 6.76 ng/mL.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Relevance of detection results between aladdin Antibody Pair (Ab176419 & Ab176421) and Roche in CLIA ·Capture antibody: sFlt-1 Mouse mAb (Ab176419) ·Detection antibody: sFlt-1 Mouse mAb (Ab176421)