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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [2]
- ELISA [1]
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- Product number
- NB600-1004 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1004, RRID:AB_10001631
- Product name
- Mouse Monoclonal VEGFR1/Flt-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy.
Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells.
Verma M, Shimizu-Motohashi Y, Asakura Y, Ennen JP, Bosco J, Zhou Z, Fong GH, Josiah S, Keefe D, Asakura A
PLoS genetics 2019 Dec;15(12):e1008468
PLoS genetics 2019 Dec;15(12):e1008468
Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells.
Ptaszynska MM, Pendrak ML, Bandle RW, Stracke ML, Roberts DD
Molecular cancer research : MCR 2008 Mar;6(3):352-63
Molecular cancer research : MCR 2008 Mar;6(3):352-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Cross-reactivity of monoclonal and polyclonal anti-human Flt-1 antibodies with mouse Flt-1. Standard ELISA assay with coating of 2.5 ug/ml mouse sFlt-1-Fc
