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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00003669-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003669-M01, RRID:AB_539940
- Product name
- Mouse Monoclonal ISG20 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ISG20 - interferon stimulated gene 20kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Antiviral activities of ISG20 in positive-strand RNA virus infections.
Intracellular activation of interferon regulatory factor-1 by nanobodies to the multifunctional (Mf1) domain.
Zhou Z, Wang N, Woodson SE, Dong Q, Wang J, Liang Y, Rijnbrand R, Wei L, Nichols JE, Guo JT, Holbrook MR, Lemon SM, Li K
Virology 2011 Jan 20;409(2):175-88
Virology 2011 Jan 20;409(2):175-88
Intracellular activation of interferon regulatory factor-1 by nanobodies to the multifunctional (Mf1) domain.
Möller A, Pion E, Narayan V, Ball KL
The Journal of biological chemistry 2010 Dec 3;285(49):38348-61
The Journal of biological chemistry 2010 Dec 3;285(49):38348-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ISG20 Antibody (1B2-3C9) [H00003669-M01] - Analysis of monoclonal antibody to ISG20 on HeLa cell . Antibody concentration 10 ug/ml.
