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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB15663 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB15663, RRID:AB_10677229
- Product name
- SFRP4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised aganist synthetic peptide of SFRP4.
- Antigen sequence
TNSSCQCPHILPHQD- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R
Genes & development 2007 Dec 1;21(23):3110-22
Genes & development 2007 Dec 1;21(23):3110-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SFRP4 polyclonal antibody (Cat # PAB15663) (0.3 ug/mL) staining of human uterus lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SFRP4 polyclonal antibody (Cat # PAB15663) (3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
