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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX113937 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113937, RRID:AB_2038168
- Product name
- TRIM32 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein by Western blot analysis.A. 50 µg rat muscle lysate/extract 7.5 % SDS-PAGETRIM32 antibody (GTX113937) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE GTX113937 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract7.5% SDS-PAGETRIM32 antibody (GTX113937) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein by western blot analysis.A. 5 ?g 293T whole cell lysate/extract B. 5 ?g whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells7.5% SDS-PAGETRIM32 antibody (GTX113937) dilution: 1:20000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein by western blot analysis.A. 5 ?g 293T whole cell lysate/extract B. 5 ?g whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells7.5 % SDS-PAGETRIM32 antibody (GTX113937) dilution: 1:10000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis.Sample: A375 cells were fixed in ice-cold MeOH for 5 min.Green: TRIM32 protein stained by TRIM32 antibody (GTX113937) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM32 antibody detects TRIM32 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TRIM32 antibody (GTX113937) diluted at 1:400.
