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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-62127 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM32 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NLTPLSVAMN CQGLIGVTDS YDNSLKVYTL DGHCVACHRS QLSKPWGITA LPSGQFVVTD VEGGKLWCFT VDRGSGVVKY SCLCSAVRPK FVTCDA Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references TRIM32 triggers β-catenin signaling through ubiquitylation of AXIN1 to promote inflammatory factor-induced apoptosis of rat nucleus pulposus cells.
Chen F, Guo Q, Chen Q, Han Z, Zhou X, Wu L, Guo X, Ni B, Yang J
American journal of physiology. Cell physiology 2020 Mar 1;318(3):C695-C703
American journal of physiology. Cell physiology 2020 Mar 1;318(3):C695-C703
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM32 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue. Samples were probed using a TRIM32 Polyclonal Antibody (Product # PA5-62127).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of TRIM32 in human cell line A-431 shows positivity in intermediate filaments. Samples were probed using a TRIM32 Polyclonal Antibody (Product # PA5-62127).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TRIM32 in human gallbladder using a TRIM32 Polyclonal Antibody (Product # PA5-62127) shows strong nuclear and cytoplasmic positivity in glandular cells.
