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- Antibody Data
- Antigen structure
- References [9]
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- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00022954-M09 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00022954-M09, RRID:AB_792411
- Product name
- Mouse Monoclonal TRIM32 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TRIM32 (2E5)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A complex of the ubiquitin ligase TRIM32 and the deubiquitinase USP7 balances the level of c-Myc ubiquitination and thereby determines neural stem cell fate specification.
The ubiquitin ligase tripartite-motif-protein 32 is induced in Duchenne muscular dystrophy.
TRIM32 modulates pluripotency entry and exit by directly regulating Oct4 stability.
Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions.
Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells.
TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration.
Nuclear factor kappa B signaling initiates early differentiation of neural stem cells.
TRIM32 promotes neural differentiation through retinoic acid receptor-mediated transcription.
The TRIM-NHL protein TRIM32 activates microRNAs and prevents self-renewal in mouse neural progenitors.
Nicklas S, Hillje AL, Okawa S, Rudolph IM, Collmann FM, van Wuellen T, Del Sol A, Schwamborn JC
Cell death and differentiation 2019 Mar;26(4):728-740
Cell death and differentiation 2019 Mar;26(4):728-740
The ubiquitin ligase tripartite-motif-protein 32 is induced in Duchenne muscular dystrophy.
Assereto S, Piccirillo R, Baratto S, Scudieri P, Fiorillo C, Massacesi M, Traverso M, Galietta LJ, Bruno C, Minetti C, Zara F, Gazzerro E
Laboratory investigation; a journal of technical methods and pathology 2016 Aug;96(8):862-71
Laboratory investigation; a journal of technical methods and pathology 2016 Aug;96(8):862-71
TRIM32 modulates pluripotency entry and exit by directly regulating Oct4 stability.
Bahnassawy L, Perumal TM, Gonzalez-Cano L, Hillje AL, Taher L, Makalowski W, Suzuki Y, Fuellen G, del Sol A, Schwamborn JC
Scientific reports 2015 Aug 26;5:13456
Scientific reports 2015 Aug 26;5:13456
Polarized and Stage-Dependent Distribution of Immunoreactivity for Novel PDZ-Binding Protein Preso1 in Adult Neurogenic Regions.
Lee ES, Kim WR, Kim Y, Lee HW, Kim H, Sun W
Endocrinology and metabolism (Seoul, Korea) 2014 Sep;29(3):349-55
Endocrinology and metabolism (Seoul, Korea) 2014 Sep;29(3):349-55
Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells.
Izumi H, Kaneko Y
Cancer research 2014 Oct 1;74(19):5620-30
Cancer research 2014 Oct 1;74(19):5620-30
TRIM32 regulates skeletal muscle stem cell differentiation and is necessary for normal adult muscle regeneration.
Nicklas S, Otto A, Wu X, Miller P, Stelzer S, Wen Y, Kuang S, Wrogemann K, Patel K, Ding H, Schwamborn JC
PloS one 2012;7(1):e30445
PloS one 2012;7(1):e30445
Nuclear factor kappa B signaling initiates early differentiation of neural stem cells.
Zhang Y, Liu J, Yao S, Li F, Xin L, Lai M, Bracchi-Ricard V, Xu H, Yen W, Meng W, Liu S, Yang L, Karmally S, Liu J, Zhu H, Gordon J, Khalili K, Srinivasan S, Bethea JR, Mo X, Hu W
Stem cells (Dayton, Ohio) 2012 Mar;30(3):510-24
Stem cells (Dayton, Ohio) 2012 Mar;30(3):510-24
TRIM32 promotes neural differentiation through retinoic acid receptor-mediated transcription.
Sato T, Okumura F, Kano S, Kondo T, Ariga T, Hatakeyama S
Journal of cell science 2011 Oct 15;124(Pt 20):3492-502
Journal of cell science 2011 Oct 15;124(Pt 20):3492-502
The TRIM-NHL protein TRIM32 activates microRNAs and prevents self-renewal in mouse neural progenitors.
Schwamborn JC, Berezikov E, Knoblich JA
Cell 2009 Mar 6;136(5):913-25
Cell 2009 Mar 6;136(5):913-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 over-expressed 293 cell line, cotransfected with TRIM32 Validated Chimera RNAi or non-transfected control. Blot probed with H00022954-M09. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of TRIM32 expression in transfected 293T cell line by TRIM32 monoclonal antibody (M09), clone 2E5. Lane 1: TRIM32 transfected lysatE (72 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TRIM32 Antibody (2E5) [H00022954-M09] - Detection limit for recombinant GST tagged TRIM32 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TRIM32 Antibody (2E5) [H00022954-M09] - Analysis of monoclonal antibody to TRIM32 on HeLa cell. Antibody concentration 10 ug/ml
