PA1-84457

antibody from Invitrogen Antibodies

Targeting: CALCR CTR

Western blot

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: PA1-84457 - Provider product page
Provider: Invitrogen Antibodies
Product name: Calcitonin Receptor Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: 4°C or -20°C if preferred

CALCR protein structure - PA1-84457 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Additional file 2: Figure S1. A, Western blot showing AMY3 transfected HEK293 cells demonstrate a marked increase in level of expression of CTR and RAMP3 proteins compared to wild-type (WT) HEK cells. B, in BV2 cells, RAMP3 protein expression shows a marked decreased after RAMP3 siRNA transfection compared to the control non-transfected cells. (JPEG 1495 kb)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Microglial cells express functional amylin receptors. a Primary cultures of human fetal microglia (HFMs) that are stained with microglial antibody, Iba-1 (green), and DyLight-594-labeled lectin (red). b These primary cultured HFMs were also stained for the two dimeric proteins that are components of the amylin receptor 3 (AMY3) subtype, the calcitonin receptor (CTR), and the receptor-associated membrane protein 3 (RAMP3). c Cultured HFMs were loaded with the fluorescent intracellular calcium dye, Fluo-8L-AM (green), and with lectin (red), an in vivo microglial marker. Arrowheads indicate cells from which Ca 2+ signals were recorded. The same cell culture is also stained with lectin (red). The field in c shows that a majority of cells are microglia. d Summary of data on intracellular calcium changes after human amylin (hAmylin) and Abeta 1-42 without and with application of the amylin receptor antagonist, AC253 in HFM (* p < 0.05, n = 123 cells in 12 culture wells from four different batch of the culture cells). The candidate traces for intracellular calcium changes are illustrated in e - i . Elevations of Ca 2+ induced by acute (30 s) application of either hAmylin (1 muM, e ) or Abeta 1-42 (1 muM, f ). The changes in Ca 2+ were blocked by AC253 (10 muM, g - i ). Traces correspond to cells identified in c with arrowheads. Scale bar = 20 mum